Journal: Biology

Article Title: Intravenous rAAV9 Produces Time-Resolved Parenchymal Labeling Downstream of the Vasculature in Adult Mice

doi: 10.3390/biology15050421

Figure Lengend Snippet: Cell lineage tracing in the periphery by intravenous rAAV9. Adult Ai9 transgenic mice received intravenous rAAV9 (CMV-CRE) injection. Tissues were harvested and imaged following 1.5, 3 and 6 months. Representative images with tdTomato reporter (Red) and Hoechst (Blue) nuclear stain were captured using an EVOS M5000 imaging system (scale bar = 125 µm).

Article Snippet: Automated quantifications of tdTomato-expressing cells on prepared tissue slides were imaged using an EVOS M5000 system (Fisher Scientific, Carlsbad, CA, USA).

Techniques: Transgenic Assay, Injection, Staining, Imaging

Journal: Biology

Article Title: Intravenous rAAV9 Produces Time-Resolved Parenchymal Labeling Downstream of the Vasculature in Adult Mice

doi: 10.3390/biology15050421

Figure Lengend Snippet: Central nervous system cell lineage tracing by intravenous rAAV9. Adult Ai9 transgenic mice received intravenous rAAV9 (CMV-CRE). Following 1.5, 3 and 6 months, tissues were harvested and imaged. Shown are representative images for tdTomato reporter (Red) and Hoechst (Blue) nuclear stain obtained with an EVOS M5000 imaging system (scale bar = 125 µm).

Article Snippet: Automated quantifications of tdTomato-expressing cells on prepared tissue slides were imaged using an EVOS M5000 system (Fisher Scientific, Carlsbad, CA, USA).

Techniques: Transgenic Assay, Staining, Imaging

Journal: Biology

Article Title: Intravenous rAAV9 Produces Time-Resolved Parenchymal Labeling Downstream of the Vasculature in Adult Mice

doi: 10.3390/biology15050421

Figure Lengend Snippet: Total tdTomato cell lineage tracing counts following intravenous rAAV9. All tdTomato-positive cells (vascular and non-vascular) were quantified by automated cell counting using ImageJ (NIH). All cardiac myocytes examined exhibited tdTomato fluorescence following AAV administration compared with control mice. Consequently, these cells were scored for fluorescence intensity in arbitrary units (a.u.) per nucleus. Other tissues were scored for the number of tdTomato-positive cells/nuclei using automated/unbiased ImageJ processing. Nuclei were labeled using Hoechst. Tissue sections were imaged using an EVOS M5000. Significance is shown (* p < 0.05; ** p < 0.03; *** p < 0.01; **** p < 0.007) using a one-way ANOVA with a post hoc Tukey test. Values that are not significant (ns) are shown.

Article Snippet: Automated quantifications of tdTomato-expressing cells on prepared tissue slides were imaged using an EVOS M5000 system (Fisher Scientific, Carlsbad, CA, USA).

Techniques: Cell Counting, Fluorescence, Control, Labeling